Scientific Studies

Unlike many other food supplements, Bimuno® is underpinned by a large and growing body of published scientific evidence.


Prebiotic evaluation of a novel Galacto-Oligosaccharide mixture (B-GOS) produced by the enzymatic activity of Bifidobacterium bifidum NCIMB 41171, in healthy humans: a randomized, double-blind, crossover, placebo-controlled intervention study


Background: Galacto-Oligosaccharides are selectively fermented by the beneficial member of the colonic microflora contributing to the health of the host.

Objective: We assessed the prebiotic potential of a novel galacto-oligosaccharide produced through the action of β-galactosidases, originating from a probiotic Bifidobacterium bifidum strain, against a galacto-oligosaccharide produced through the action of an industrial β-galactosidase and a placebo.

Design:Fifty-nine healthy human volunteers participated in this study. Initially, the effect of the matrix on the prebiotic properties of a commercially available galacto-oligosaccharide (7g/d) was assessed during 7 day treatment periods and a 7 day washout period in between. During the second phase, 30 volunteers were assigned to a sequence of treatments (7 days) differing in the amount of the novel galacto-oligosaccharide (0, 3.6, or 7g/d). Stools were recovered before and after each intervention, and bacteria numbers were determined by fluorescent in situ hybridization.

Results: Addition of the novel galactooligosaccharide mixture significantly increased the bifidobacterial population ratio compared with the placebo (P<0.05), whereas 7 g/d of the novel galacto-oligosaccharide significantly increased the bifidobacterial ratio compared with the commercial galacto-oligosaccharide (P<0.05). Moreover, a significant relation (P<0.001) between the bifidobacteria proportion and the novel galacto-oligosaccharide dose (0, 3.6, and 7g.d) was observed. This relationship was similar to the effect of the novel galacto-oligosaccharide on the prebiotic index of each dose.

Conclusions:This study showed that the galacto-oligosaccharide mixture produced with different β-galactosidases show different prebiotic properties and that, by using enzymes originating from bifidobacterial species, an increase in the bifidogenic properties of the prebiotic product is achievable.


Depeint F et al. Am J Clin Nutr 2008;87:785-91




Back to Scientific Studies


This section is an information and educational resource for healthcare professionals, scientists, researchers and journalists. It is not intended for consumers. If you wish to purchase Bimuno please click here

Back to the top